Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…

Deanna Hurum, Lipika Basumallick, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Prostate-Specific Antigen (PSA), Transferrin, N-Glycans, O-Glycans, Dionex CarboPac Column Introduction Changes in protein glycosylation are frequently studied in cancer research to identify potential biomarkers. Factors…

Technical Note 36 Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD INTRODUCTION Analysis of protein glycosylation is an important part of glycoprotein characterization. Oligosaccharides can be linked to a protein through a serine or a threonine as O-linked glycans,…

Application Note 215 Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column Introduction High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a widely used technique for determining an extensive set of carbohydrates including, but…