Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column

APPLICATION NOTE 72914 HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual eluent generation cartridges Authors Beibei Huang and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal Demonstrate Dual EGC capability and performance for profiling N-linked oligosaccharides released from…

TECHNICAL NOTE 72264 HPAE-PAD N-linked oligosaccharide profiling of IgG Authors Sachin Patil, Deanna Hurum, Lipika Basumallick, and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Goal To develop an HPAE-PAD method to profile the major neutral and charged asparagine-linked (N-linked) oligosaccharides…

Deanna Hurum, Lipika Basumallick, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Prostate-Specific Antigen (PSA), Transferrin, N-Glycans, O-Glycans, Dionex CarboPac Column Introduction Changes in protein glycosylation are frequently studied in cancer research to identify potential biomarkers. Factors…

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…