Best Practice for Nucleic Acid Thermal Stability Measurements Using the Cary 3500 UV-Vis Spectrophotometer

Application Note Biopharmaceuticals Fast Determination of Thermal Melt Temperature of Double-Stranded Nucleic Acids by UV-Vis Spectroscopy Reproducible melting temperature (Tm) data at fast ramp rates using the Agilent Cary 3500 UV-Vis Authors Wesam Alwan and Mathieu Rault Agilent Technologies, Inc.…

Application Note Biopharmaceuticals Characterization of Protein Thermal Stability Using UV-Vis Spectroscopy Benefits of the Agilent Cary 3500 UV‑Vis spectrophotometer with a Peltier temperature‑controlled multicell in protein therapeutic research Authors Carlos Santos Martin La Trobe Institute for Molecular Science (LIMS), La…

Application Note Academic Phase Diagram of the Ouzo Effect Using Temperature-Controlled UV-Vis Spectroscopy Simultaneous temperature ramping experiments using the Agilent Cary 3500 UV-Vis and the multizone feature of the Multicell sampling module Authors Introduction Marc-André Gagnon and Claire Cislak Agilent…

Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…