Relative Quantification of TMT-labeled, cross-linked proteins using XlinkX node in Proteome Discoverer

proteomics WHITE PAPER 65343 Crosslinking mass spectrometry (XL-MS) goes mainstream Mass spectrometrists and structural biologists guide to using XL-MS to understand the structure and function of molecular machines Author Executive summary Julian Saba, Thermo Fisher Scientific, San Jose, CA, USA…

Evaluation of FAIMS Technology for Mass Spec Analysis of Chemical Cross-linked Peptides Rosa Viner1; Leigh A Foster2; Ryan D. Bomgarden2; Michael W. Belford1; Satendra Prasad1; Romain Huguet1; Eloy R. Wouters1 , 1Thermo Fisher Scientific, San Jose, CA; 2Rockford, IL ABSTRACT…

Crosslinking mass spectrometry Optimized quantitative XL-MS Analysis on an Orbitrap Tribrid Apex mass spectrometer Yi He, Nicolas Hartal, Graeme McAlister, Rafael Maleni, Weijing Liu Thermo Fisher Scientific, San Jose, CA Abstract Results Purpose: To optimize the quantitative XL-MS workflows with…

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…