Evaluation of factors affecting detection of host cell proteins in biotherapeutic proteins using an Orbitrap Fusion Lumos Tribrid mass spectrometer

APPLICATION NOTE 73412 Residual host cell protein analysis of NISTmAb: From simplified sample preparation to reliable results Authors: Amy Claydon, Phil Widdowson, and Andrew Williamson Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Host cell protein assay, HCP, monoclonal antibody, mAb,…

APPLICATION NOTE 21918 Easy, fast and reproducible analysis of host cell protein (HCP) in monoclonal antibody preparations Authors Giorgio Oliviero1, Ken Cook 2, Kai Scheffler3, Florian Füssl1, Jonathan Bones1 1National Institute for Bioprocessing Research and Training, Dublin, Ireland; 2Thermo Fisher…

APPLICATION NOTE 74109 Comprehensive identification and label-free quantitation of host cell protein contaminants using BioPharma Finder 4.1 software Authors: Silvia Millán-Martín,1 Sara Carillo,1 Jonathan Bones1, Jennifer Sutton2 National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland 1 2 Thermo…

Confident LC-MS Identification of Low ppm Host Cell Proteins (HCPs) in Biotherapeutic Monoclonal Antibodies Amy J Claydon1, Phil Widdowson1, Andrew Williamson2, and Min Du3, Thermo Fisher Scientific, 1Runcorn, UK, 2Hemel Hempstead, UK, 3Cambridge, MA, USA Label-free quantification of NISTmAb HCPs…