Agilent AdvanceBio HIC column - User guide

High-resolution separation of ADC, showing DAR profile Column Operating parameters AdvanceBio HIC, 4.6 x 100 mm, 3.5 µm Eluent B t sulfate, 2M ammonium en ipi 50 mMxcsodium phosphate, pH 7.0 E b 50 mM sodium phosphate, mA pH 7.0…

Agilent-NISTmAb Intact Analysis Using Hydrophobic Interaction Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test includes aggregate…

Quick Start Guide AdvanceBio HIC Columns In this document, Agilent applications chemists share their recommendations for an optimum LC system and its configuration for performing hydrophobic interaction chromatography (HIC). They offer guidance on generic methods for protein and monoclonal antibody…

Why Use HILIC and HIC Chromatography? Theory and method development strategies Mohit Patel, PhD LC Applications Engineer October 8, 2024 1 10/08/2024 DE-001530 AGILENT CONFIDENTIAL Agenda 1 What is HILIC and when should you consider it? 2 How to approach…