Subunit Level Top-down ECD Characterization of Biotherapeutic Proteins: Improving Mass Spectral Clarity with Fragment Ion Level Ion Mobility

In-Depth Versatile Characterization of Aspartic Acid Isomers via a Cyclic Ion Mobility Mass Spectrometry (cIMS) Samantha Ippoliti1; Brad J. Williams1, Dale Cooper-Shepherd2, Ying Qing Yu1, Weibin Chen1, Barbara Sullivan1, James I. Langridge2 1Waters Corporation, Milford, MA, USA; 2Waters Corporation, Wilmslow,…

EXPANDING NATIVE MASS SPECTROMETRY CAPABILITIES FOR SOLUBLE AND MEMBRANE PROTEINS USING A QUADRUPOLE-ION MOBILITY-TIME-OF-FLIGHT MASS SPECTROMETRY SYSTEM Brad Williams1, Dale A. Cooper-Shepherd2, Mario Hensen3, Kleitos Sokratos3, Jonathan T.S. Hopper3, and James I. Langridge2 1. Waters Corporation, 34 Maple Drive, Milford,…

Application Note Electron Capture Dissociation in Combination With High Performance Cyclic Ion Mobility Separation for Detailed Protein Studies Dale Cooper-Shepherd Waters Corporation This is an Application Brief and does not contain a detailed Experimental section. Abstract Electron capture dissociation (ECD)…

Increasing sensitivity in peptide-level studies; Cyclic ECD product ions Dale A. Cooper-Shepherd1, Isabella A. Jones1, Emma Marsden-Edwards1, Ramin Rabbani2 1Waters Corporation, Wilmslow, Cheshire, UK; 2Waters Corporation, Milford, MA, USA IMS-enabled Wideband Enhancement of CID and INTRODUCTION OPTIMIZATION OF CYCLIC IMS…