High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring

Michael Menz, Carsten Paul, Evert-Jan Sneekes Thermo Fisher Scientific, Germering, Germany Key Words Method Transfer, Thermo Scientific ProPac WCX-10, Biocompatible UHPLC, Protein Digest, Biotherapeutics Characterization, Biopharma, Deamidation Goal Demonstrating a successful transfer of an HPLC protein deamidation monitoring method from…

Application Note 125 Monitoring Protein Deamidation by Cation-Exchange Chromatography Introduction A common structural modification of recombinant proteins is the deamidation of asparagine (Asn) residues.1,2 This modification occurs in a variety of protein-based pharmaceuticals, including human growth hormone, tissue plasminogen activator,…

3µm Monodisperse IEX Media 3µm monodisperse particles for Ion Exchange Chromatography stationary phases and their impact on the separation of biomolecules S. Bechler , K. Ma, R. Olivares, R. Cowley, Y. Hu, Y. Singh, and S. Lin, Thermo Fisher Scientific,…

Application note | 002243 Biopharma Coupling ion exchange chromatography with mass spectrometry to characterize charge heterogeneity of mAbs under near-native conditions using a ProPac 3R SCX column Application benefits Authors Sensen Chen , Xiaoxi Zhang , 1 1 Christof Mitterer2,…