Transferring a Method for the Characterization of PTMs from the UltiMate 3000 BioRS System to the Vanquish Flex UHPLC System

Table of contents Introduction White Paper 72711 An instrument parameter guide for successful (U)HPLC method transfer Technical Note 73371 Physical adjustment of gradient delay volume as a tool for successful transfer of HPLC methods Application Note 73186 Improving peak results…

Michael Menz, Carsten Paul, Evert-Jan Sneekes Thermo Fisher Scientific, Germering, Germany Appli cat i on Br i e f 7 1 8 1 1 High Salt Gradient Analysis of Post-Translational Modifications Deamidation Monitoring Key Words Biopharma, biopharmaceuticals, Vanquish Flex UHPLC,…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

WHITE PAPER 72711 An instrument parameter guide for successful (U)HPLC method transfer Authors Carsten Paul, Maria Grübner, Michael Heidorn, Matthias Krajewski, Sabrina Patzelt, Thomas Piecha, and Frank Steiner Thermo Fisher Scientific, Germering, Germany Keywords UHPLC, HPLC, USP <621>, gradient delay…