Leveraging Bottom-Up Search Results to Build Sample Specific Top-Down Databases

The intelligent protein informatics platform Thermo Scientific Proteome Discoverer software Transform proteomics mass spectrometry data into insights Thermo Scientific™ Proteome Discoverer™ software enables comprehensive proteomics data processing workflows empowered by artificial intelligence. • Powerful and flexible framework: Optimized analysis for…

Improvements to ProSightPD nodes in the Thermo Scientific Proteome Discoverer Software Framework David M. Horn,1 Tara L. Schroeder,1 Ioanna Ntai,1 Richard D. Leduc,2 Ryan T. Fellers,2 Joseph B. Greer,2 and Neil L. Kelleher2, 1Thermo Fisher Scientific, San Jose, CA, 2Northwestern…

High-throughput Top-down FAIMS Data Analysis with ProSightPD Nodes in Proteome Discoverer Software Mick Greer1, Vincent Gerbasi2, Joseph Greer3, Ryan Fellers3, David Horn4, Romain Huguet4, Susan Abbatiello5, Michael Belford4, Philip Compton2, Kenneth Durbin3, Neil Kelleher2, Richard LeDuc3, Scott Peterman4, Paul Thomas2…

For bottom-up experiments, the monoclonal antibodyy Rituximab was denatured for 30 min in 7 M Urea and 50 mM Tris HCL at pH 8.00. The sample was reduced with 5 mM 37°C and alkylated by addition of 10 mM IAA…