Methods for the Anion Exchange Chromatographic Analysis of mRNAs

Application Note Salt Plug Injection Methods for Improved Anion Exchange Analyses of Large Nucleic Acids Szabolcs Fekete, Matthew A. Lauber Waters Corporation Abstract With a burgeoning pipeline of nucleic acid-based therapies, there is a need for improved analytical methods that…

FAST AND HIGH RECOVERY ANALYTICAL CHARACTERIZATION OF mRNAs USING WEAK ANION EXCHANGE AND ION PAIRING REVERSED PHASE CHROMATOGRAPHY Mateusz IMIOLEK1, Szabolcs FEKETE1, Matthew A. LAUBER2 1Waters Corporation, Geneva, Switzerland, 2Waters Corporation, Milford, MA, USA INTRODUCTION ANION EXCHANGE CHROMATOGRAPHY (AEX) AEX…

Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size Exclusion Chromatography-Multi Angle Light Scattering, and Charge Detection Mass Spectrometry Patrick Brophy1; Henry Shion1; Catalin E Doneanu1; Hua Yang1; Benjamin Draper2; Daniel Botamanenko2; Susan Abbatiello1; Andy Jarrell1; Stephan Koza1;…

Application Note Size and Purity Assessment of Single-Guide RNAs by Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract Single-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size…