Exploring high complexity proteomes with a DIA based quant and ID workflow

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

US HUPO 2024 Deep proteome coverage at scale combined with reproducible quantitation on the timsTOF HT Stephanie Kaspar-Schoenefeld1, Andreas Schmidt1, Markus Lubeck1, Pierre-Olivier Schmit2, Torsten Mueller1 and Narayanaganesh Balasubramanian3 (A) (B) Fig. 1: Reproducible and in-depth protein identification from yeast…

Unleashing the power of HT-DIA acquisition on Orbitrap Exploris 240 MS – Precise and accurate quantitation at 260 SPD Dominic G. Hoch1, Riccardo Stucchi1, Jeff op de Beeck2, Julia Krägenbring3, Ece Aydin4, Maciej Bromirski5 1Thermo Fisher Scientific, Reinach, Switzerland; 2Thermo…

Accurate Label-Free Protein Quantitation on the timsTOF Pro with 4D-Proteomics™ The timsTOF Pro platform brings together two unique technologies, namely Trapped Ion Mobility Spectrometry (TIMS) mode operation at the front-end and Parallel Accumulation Serial Fragmentation (PASEF [1]) for data acquisition.…