Agilent AdvanceBio SEC 1.9 μm Columns - User guide

Operating parameters Parameter Mobile Phase Compatibility pH Stability Value 150 mM phosphate buffer, pH 7.0 (recommended starting conditions) Other aqueous buffers with high and low salt can be used. Mixtures of water and acetonitrile can be used. Recommended starting conditions…

Consumables Workflow Ordering Guide Analyzing Monoclonal Antibody (mAb) Fragments and Dimers Using Size Exclusion Chromatography (SEC) Low molecular weight (LMW) fragments and high molecular weight (HMW) dimers and aggregates can be formed from biotherapeutic proteins during drug development, storage, shipment,…

Consumables Workflow Ordering Guide Analyzing Monoclonal Antibody (mAbs) and mAb-Derived Therapeutics Using Native SEC-MS The quantitation of aggregates is of particular concern for protein-based-therapies given their potential effect on efficacy and immunogenicity. Size exclusion chromatography (SEC) combined with UV detection…

Consumables Workflow Ordering Guide Improving the Quality of Antibody Drug Conjugates by Orthogonal Analytical Methods Antibody Drug Conjugates Antibody-drug conjugates (ADCs) represent a new generation of targeted biotherapeutics that make up a rapidly growing segment of the drug discovery pipeline.…