Improving the Quality of Antibody Drug Conjugates by Orthogonal Analytical Methods

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…

Consumables Workflow Ordering Guide Analyzing Monoclonal Antibody (mAbs) and mAb-Derived Therapeutics Using Native SEC-MS The quantitation of aggregates is of particular concern for protein-based-therapies given their potential effect on efficacy and immunogenicity. Size exclusion chromatography (SEC) combined with UV detection…

Application Note Biopharma/Pharma Characterization of Antibody‑Drug Conjugates Using 2D-LC and Native MS Authors David L. Wong and Sarah M. Stow Agilent Technologies, Inc. Abstract Antibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb) conjugated to a small molecule drug…

Analysis of Cysteine-Linked Antibody Drug Conjugates Using Hydrophobic Interaction Chromatography on the Agilent 1260 Infinity II Bio-inert LC Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Hydrophobic interaction chromatography (HIC) is frequently used for the Agilent Technologies, Inc. determination…