Characterizing Weak Binding Events with Two Advanced Biophysical Techniques: Hydrogen Deuterium Exchange (HDX-MS) and Differential Scanning Calorimetry (DSC)

LOCALIZED CONFORMATION ANALYSES OF MUTATED HUMAN IgG1 BY HYDROGEN DEUTERIUM EXCHANGE MASS SPECTROMETRY AND DIFFERENTIAL SCANNING CALORIMETRY Joomi Ahn 1, Xiaojun Lu2, Colette Quinn3, St John Skilton1, Ying Qing Yu1, and Jihong Wang2 1 Waters Corporation, 2 MedImmune, 3TA Instruments…

[ BIOPHYSICAL CHARACTERIZATION ] Stability Workflow in Biopharmaceuticals Biologic drugs are susceptible to compromised structural and conformational stability and these changes ht ps:/ www.waters.com/waters/en_US/Hydrogen-Deuterium-Exchange-with-Mas -Spec-and-HDX-MS/nav.htm?locale=en_US&cid=10185909 can lead to loss of activity. Building from our HDX LC-MS, and through our TA…

Conformational Characterization of Calmodulin by Hydrogen Deuterium Exchange Mass Spectrometry Joomi Ahn, Martha Stapels, Michael Eggertson, Keith Fadgen, and Ying Qing Yu Waters Corporation, Milford, MA, U.S. A P P L I C AT I O N B E N…

WHITE PAPER 65872 Hydrogen deuterium exchange mass spectrometry for the masses Executive summary Determining the shape and dynamics of proteins and protein complexes is crucial to understanding protein function and the roles proteins play in biological systems. Traditional techniques employed…