Impact of Reversed-Phase Chiral Chromatography on the LC-MS Analysis of Drugs in Biological Fluids

Utilizing Enantiomeric Separations in Bioanalysis Craig R. Aurand, Hillel K. Brandes, David S. Bell, Wayne Way, and Carmen T. Santasania Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA www.sigma-aldrich.com T410094 Abstract Enantiomeric separations are often restricted in bioanalysis due to…

Basics of Chiral HPLC Definitions Principles Available CSPs Mobile phase types T408109 sigma-aldrich.com The Field of Stereochemistry 1. All isomers have same chemical formula but differ in the arrangement of certain chemical groups in space. 2. Many types of isomers…

HPLC Enantiomeric Separations of Pharmaceuticals Using Polar Organic Mobile Phases •J.T. Lee and William Campbell Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA sigma-aldrich.com T411054 Agenda • • • • • • • • • Background Benefits Mechanisms Separation Comparisons…

A Novel Approach in HPLC Chiral Method Development: Dealing with Multiple Chiral Centers David S. Bell1, Anders Fridstrom2, and Gaurang Parmar1 1Supelco, Div. of Sigma-Aldrich Bellefonte, PA 16823 USA 2Sigma-Aldrich GmbH Industriestrasse 25 CH-9471 Buchs, Switzerland sigma-aldrich.com/analytical T413140 © 2013…