Comparing Mobile Phase Additives for the Separation of mAb Tryptic Peptides: A Case Study on Formic, Difluoroacetic, and Trifluoroacetic Acid

UNIVERSITY OF COPENHAGEN FA C U LT Y O F S C I E N C E Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing Jennifer M. Nguyen1,2, Xiaoxiao Liu2, and Matthew A. Lauber2 1University of Copenhagen, Frederiksberg,…

Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing Jennifer Nguyen, Xiaoxiao Liu, and Matthew A. Lauber Waters Corporation, Milford, MA, USA INTRODUCTION Peptide mapping is an important and critical technique in the characterization of biopharmaceuticals. These separations are…

[ APPLICATION NOTE ] Selecting a Reversed-Phase Column for the Peptide Mapping Analysis of a Biotherapeutic Protein Stephan M. Koza and Erin E. Chambers Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Side-by-side performance of ten The peptide mapping…

Peptide Mapping for Biotherapeutics Strategies for Simplifying Protein Digestion A sponsored publication from Peptide Mapping Doesn’t Need to Be Complex Introducing PeptideWorks™ Tryptic Protein Digestion Kits PeptideWorks Tryptic Protein Digestion Kits uniquely deliver automatable, high efficiency, reproducible peptide maps in…