Impact of LC System Dispersion on the Size-Exclusion Chromatography Analysis of Monoclonal IgG Antibody Aggregates and Fragments: Selecting the Optimal Column for Your Method

[ APPLICATION NOTE ] High Resolution and High Throughput Size-Exclusion Chromatography Separations of IgG Antibody Aggregates and Fragments on UHPLC and HPLC Systems with 2.5 µm BEH Particles Stephan M. Koza and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION…

[ APPLICATION NOTE ] Evaluating the Impact of LC System Dispersion on the Size-Exclusion Chromatography Analysis of Proteins Stephan M. Koza, Corey E. Reed, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Understanding the measurement Size-exclusion…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

[ CARE AND USE MANUAL ] BioResolve SEC mAb Guard and Columns CONTENTS I. INTRODUCTION II. SEC ANALYSIS OF MONOCLONAL IgG ANTIBODY AGGREGATES, MONOMERS, AND FRAGMENTS a. Considerations b. Determining Extra-Column LC System Dispersion c. Selecting the Appropriate …