Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion‑Pair Reverse Phase Chromatography

Application Note Metabolomics Discovery Metabolomics LC/MS Methods Optimized for Polar Metabolites Authors Yuqin Dai and Jordy J. Hsiao Agilent Technologies, Inc. Abstract High pH and low pH hydrophilic interaction chromatography (HILIC) methods were developed to provide comprehensive coverage of many…

Application Note Metabolomics Monitoring of Mammalian Cell Culture Media with HILIC LC/MS Authors Jordy J. Hsiao, Genevieve Van de Bittner, Te‑Wei Chu, Oscar G. Potter, and Hongfeng Yin Agilent Technologies, Inc. Abstract An Agilent InfinityLab Poroshell HILIC-Z column, in combination…

Application Note Biopharma/Pharma Improved Metabolomic Analysis Using an Iron-Free Flow Path Comparing the Agilent 1290 Infinity II Bio LC System and Agilent 1290 Infinity II LC System for metabolomics ×105 ATP 4.5 4.0 Agilent 1290 Infinity II Bio LC Agilent…

C146-E280D Selection Guide Metabolite Analysis Metabolomics Product Portfolio Expanding Metabolomics Metabolomics refers to an array of techniques used to comprehensively detect and analyze various metabolites formed in vivo during biological activity. The qualitative and quantitative changes in metabolites reflect the…