Calibrating your Agilent AdvanceBio SEC Columns

Agilent “How to” Guide for SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SEC The chromatographic separation of biomolecules based on their size in solution is known as size exclusion chromatography (SEC). Unlike other modes of chromatography, it…

Agilent Biocolumns Aggregate/Fragment Analysis Application Compendium Contents Background 2 Getting Started 3 How to Guide - Size Exclusion Chromatography for Biomolecule Analysis - 5991-3651EN 4 Featured Application Notes Elevate Your mAb Aggregate Analysis - 5994-2709EN 1 22 22 High-Resolution, High-Throughput…

Choosing the Right Calibration for the Agilent Bio SEC-3 Column Application Note Biotherapeutics & Biosimilars Author Introduction Keeley Mapp Size exclusion chromatography (SEC) is a technique for separating molecules by size using aqueous eluents. This typically includes molecules such as…

Operating parameters Parameter Mobile Phase Compatibility pH Stability Value 150 mM phosphate buffer, pH 7.0 (recommended starting conditions) Other aqueous buffers with high and low salt can be used. Mixtures of water and acetonitrile can be used. Recommended starting conditions…