High Resolution Size-Exclusion Chromatography Separations of mAb Aggregates, Monomers, and Fragments using BioResolve SEC mAb Columns on UPLC, UHPLC, and HPLC Chromatography Systems

[ CARE AND USE MANUAL ] BioResolve SEC mAb Guard and Columns CONTENTS I. INTRODUCTION II. SEC ANALYSIS OF MONOCLONAL IgG ANTIBODY AGGREGATES, MONOMERS, AND FRAGMENTS a. Considerations b. Determining Extra-Column LC System Dispersion c. Selecting the Appropriate …

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

[ APPLICATION NOTE ] Impact of LC System Dispersion on the Size-Exclusion Chromatography Analysis of Monoclonal IgG Antibody Aggregates and Fragments: Selecting the Optimal Column for Your Method Stephan M. Koza, Corey E. Reed, and Weibin Chen Waters Corporation, Milford,…

Application Note Comparison of Sub-3-µm HP-SEC Columns for the Analysis of IgG Antibody Aggregates (HMWS) and Fragments (LMWS) Stephan M. Koza, Weibin Chen Waters Corporation This is an Application Brief and does not contain a detailed Experimental section. Abstract The…