LC/MS Analysis of the Monoclonal Antibody Rituximab Using the Q Exactive Benchtop Orbitrap Mass Spectrometer

RES MS analysis of antibodies at the protein and peptide levels is critical during development and production of biopharmaceuticals. The compositions of current generation therapeutic proteins are often complex due to their heterogeneity caused by various modifications which are relevant…

Application Note Biopharma/Pharma Rituximab Biosimilar Analysis Using the Agilent InfinityLab Pro iQ Plus LC/MS Author Abstract Ravindra Gudihal Agilent Technologies, Inc. The biotherapeutics field is rapidly expanding, with recombinant monoclonal antibodies (mAbs) emerging as key products, particularly in the development…

APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…

experiments were performed yielding 100% sequence coverage, site specific information of the drug-conjugate as well as expected and unexpected modifications. 10 21 0 Methods Sample Preparation M N LC Poster Note 643 97 The T-DM1 sample was reduced by DTT…