Two-Dimensional Liquid Chromatography Isolation and Quantitation of IgG and Exosomes from Cell Culture Media

Application Note Biopharma/Pharma Determination of Multiple Attributes of Monoclonal Antibodies Simultaneous and parallel multi-attribute analysis using 3D-LC/MS with 2D multimethod option mAb SEC Size variants Protein A CEX LC/MS mAb Titer Charge variants Mol wt (MW) AA sequence PTM Cell…

Application note | 004276 Biopharma Monitoring monoclonal antibody bioproduction processes with 2D-LC-UV Author Application benefits Maria Grübner, Thermo Fisher Scientific, • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of cell culture samples enables titer determination and purification for subsequent…

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…

A novel approach for multiple PQAs monitoring of mAbs from bioreactors using 2D-LC-MS Maria Grübner a, Christof Mitterer b, Alexander Schwahn c, Aleš Holfeld c, Sara Carillo e, Ken Cook d, Jonathan Bones e a-d Thermo Fisher Scientific (a Germering/Germany;…