Proteome wide interactomics analysis using MS-cleavable crosslinkers and the Orbitrap Astral Zoom mass spectrometer

Optimization of FAIMS-XL-MS Workflow for Phospho-Enrichable Crosslinkers Yuqi Shi1, Brand D. Groppe2, Leigh Foster3, Ryan Bomgarden3, Rosa Viner1, Thermo Fisher Scientific, 1San Jose, CA, 2Kansas City, MO, 3Rockford, IL Previously we described optimized FAIMS XL-MS workflow for instruments with ion-funnel…

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…

LC separation optimization for XL-MS Analysis Yi He1, Erum Raja2, Leigh Foster2, Max Ruwolt3, Anthony Ciancone4, Fan Liu3, Francis O’Reilly4, Ryan Bomgarden2, Rosa Viner1 1Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Rockford, Illinois, USA; 3Department of Structural…

evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…