Charge variant analysis of cysteine-linked antibody-drug conjugates using an online multiple heart-cut 2D- SCX-SEC-LC HRAM MS approach

Biopharma High-sensitive multi-attribute analysis of ADCs under native conditions by using an online multiple heart-cut 2D-LC-HRAM mass spectrometry system Frank Steiner1, Maria Gruebner1, Xuepu Li2, Xiaoxi Zhang2, Min Du3 1Thermo Fisher Scientific, Germering, Germany, 2Thermo Fisher Scientific, Shanghai, China, 3Thermo…

High Resolution Mass Spectrometry of Antibody Drug Conjugates Using the Orbitrap Mass Analyzer Kai Scheffler1, Eugen Damoc2, Aaron Bailey3, and Jonathan Josephs3 Thermo Fisher Scientific, 1Dreieich, Germany, 2Bremen, Germany, 3San Jose, USA Figure 1: Operating modes for the three major…

A comprehensive CE/ESI-MS solution for BioPharma applications Thermo Scientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometers with ZipChip system Fast CE Separation Nanospray Sensitivity HRAM Spectrometry A unique and powerful combination of CE/ESI and MS technology designed for BioPharmaceutical applications Thermo…

Comprehensive Characterization of Cysteine-conjugated Antibody Drug Conjugate (ADC) on a Hybrid Quadrupole-Orbitrap Mass Spectrometer Xiaoxi Zhang1, Kristina Srzentić2 and Min Du2, Thermo Fisher Scientific 1 Shanghai, China; 2 Cambridge, Massachusetts, US Purpose: In-depth characterization of an antibody cysteine-fluorophore conjugate standard…