High-sensitivity Host Cell Protein (HCP) analysis on a new Orbitrap Excedion Pro BioPharma hybrid mass spectrometer with optimized sample preparation

Increasing sensitivity of high throughput host cell protein analysis on a novel highresolution accurate mass platform Eugen Damoc1; Tabiwang N. Arrey1; Anna Pashkova1; Eduard Denisov1; Kai Scheffler2; Kristina Srzentić3 1Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany; 2Thermo Fisher Scientific, Germering,…

Enhanced HCP quantitation LC-MS/MS workflow solution using a MS sample prep method Jae Choi1, Reiko Kiyonami2, Min Du2, Sarah Driscoll3, Km Shams Ud Doha3, Nikki Jarrett1, Kay Opperman1, Ryan Bomgarden1, Bhavin Patel1; 1Thermo Fisher Scientific, Rockford, IL, 2Thermo Fisher Scientific,…

Applying UHPLC HRAM MS-MS/MS method to assess host cell protein clearance during the purification process development of therapeutic mAbs Reiko Kiyonami1, Ying Chen2, AI De Leon2, Min Du1, 1Thermo Fisher Scientific, Lexington, MA; 2Thermo Fisher Scientific, Bedford, MA Introduction Mass…

APPLICATION NOTE 73412 Residual host cell protein analysis of NISTmAb: From simplified sample preparation to reliable results Authors: Amy Claydon, Phil Widdowson, and Andrew Williamson Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Host cell protein assay, HCP, monoclonal antibody, mAb,…