An Automated Sample Preparation Protocol to Determine the Encapsulation Efficiency of RNA-Loaded Lipid Nanoparticles Using Andrew+ Pipetting Robot

Application Note Biopharmaceuticals Determination of mRNA Encapsulation Efficiency with the Agilent 1290 Infinity II Bio LC System Authors Aveline Neo, Suresh Babu C.V., and Zhi Ting Teo Agilent Technologies, Inc. Li Zhang and Yi Yan Yang Bioprocessing Technology Institute, Agency…

APPLICATION NOTE Skip the mess with dye-free LNP payload quant on Stunner Introduction Everyone who’s worked with lipid nanoparticles (LNPs) has used a fluorescent dye-based assay to quantify the RNA content and encapsulation efficiency (EE%) of their formulations and production…

APPLICATION NOTE Flexible nanoparticle development: process optimization & scale-up of a wide range of synthetic nanomedicines with Sunshine Introduction In contrast, by using microfluidic technology to combine reactants in a precise mixing geometry using stable, highly controlled, laminar flows, the…

APPLICATION NOTE Process optimization and pre-clinical production of lipid nanoparticles using Sunshine Introduction optimizing the conditions for producing nanoparticles can be laborious and resource intensive. For instance, ethanol injection is commonly employed to synthesize mRNA-LNPs, in which lipids suspended in…