HPAE-PAD analysis of N-linked glycans: improving glycan resolution

APPLICATION NOTE 73063 Improved profiling of sialylated N-linked glycans by ion chromatography-Orbitrap mass spectrometry Authors Sachin Patil and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords HPAE-PAD, glycoprotein, sialylated glycans, Q Exactive, HPAE-MS, HRAM, CarboPac PA200 column Goal To describe…

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…

TECHNICAL NOTE 70671 Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Author: Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Technical Note 20, monosaccharide, alditol, glycan, oligosaccharide, sialic acid, sugar Goal Update Dionex Technical Note 20 to…

TECHNICAL NOTE 70671 Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Author: Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Technical Note 20, monosaccharide, alditol, glycan, oligosaccharide, sialic acid, sugar Goal Update Dionex Technical Note 20 to…