Evaluation of Modern and Legacy HPLC Systems for the Size-Exclusion Analysis of Aggregation and Fragmentation in a Monoclonal Antibody Drug

ADVANCING ANALYTICAL APPROACHES FOR ROBUST EVALUATION OF LARGE MOLECULE BIOLOGICS USING LIQUID CHROMATOGRAPHY Corey E. Reed, Andrew Steere, Paula Hong Waters Corporation, Milford, MA 01757 (USA) INTRODUCTION • RESULTS & DISCUSSION Size-Exclusion Chromatography (SEC) and Ion-Exchange Chromatography (IEX) are prominent…

[ APPLICATION NOTE ] Impact of LC System Dispersion on the Size-Exclusion Chromatography Analysis of Monoclonal IgG Antibody Aggregates and Fragments: Selecting the Optimal Column for Your Method Stephan M. Koza, Corey E. Reed, and Weibin Chen Waters Corporation, Milford,…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

[ APPLICATION NOTE ] Evaluating the Performance of the ACQUITY Arc Bio UHPLC System for the Size-Exclusion Chromatography Analysis of a Monoclonal Antibody Corey E. Reed, Stephan M. Koza, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■…