Tunable Digestions of RNA Using RapiZyme™ RNases to Confirm Sequence and Map Modifications

sgRNA AND mRNA SEQUENCE MAPPING VIA ENDONUCLEASE DIGESTION AND LC-MS ANALYSIS, USING NOVEL INFORMATICS WORKFLOWS Catalin Doneanu1, Alexandre F. Gomes1, Chris Preston2, Matt Gorton2, Tatiana Johnson1, Bala Addepalli1, Heidi Gastall2 and Ying Qing Yu1 1Waters Corporation, Milford, MA, USA; 2Waters…

Application Note RNA Digestion Product Mapping Using an Integrated UPLC-MS and Informatics Workflow Catalin E. Doneanu, Chris Preston, Matt Gorton, Tatiana Johnson, Bala Addepalli, Ying Qing Yu Waters Corporation Abstract LC-MS analysis is an indispensable tool for single-guide RNA (sgRNA)…

Application Note Digesta Ex Machina: Automating Sample Preparation for Oligo Mapping With Rapizyme™ MC1 and Cusativin on Andrew+™ Robot Tatiana Johnston, Christian Reidy, Balasubrahmanyam Addepalli Waters Corporation Abstract This study was designed to demonstrate the suitability of the Andrew+ Pipetting…

Using Novel RNases to Measure 5’ Cap and Poly(A) Tail Modifications during Oligonucleotide Mapping LC-MS of messenger RNA Authors: Balasubrahmanyam Addepalli; Tatiana Johnston; Catalin Doneanu; Alexandre Gomes; Christian Reidy; Ying Qing Yu; Matthew Lauber Affiliations: WATERS CORPORATION, MILFORD MA 01757…