Peptide Mapping of Tryptic Digests for mAbs using a novel ECD cell on the 6545XT AdvanceBio LC/Q-TOF Mass Spectrometer

Application Note Peptide Mapping of Trastuzumab Tryptic Digests on an Agilent 6545XT AdvanceBio LC/Q-TOF Author Stephen Sciuto, PhD Agilent Technologies, Inc. Abstract This application note describes a peptide mapping strategy to analyze tryptic digests of trastuzumab on an Agilent 6545XT…

Transformative Workflows for Protein Characterization: ECD and Multidimensional Separations Expert Insights Sponsored by Expert Insights Contents Introduction 3 The Role of Electron Capture Dissociation in Biomolecular Analysis Adapted from Cooper, et al. 4 Offline Coupling of Hydrophobic Interaction Chromatography– Capillary…

Application Note Biotherapeutics and Biosimilars Glycopeptide Characterization for Various Monoclonal Antibodies Using the Agilent 6545XT AdvanceBio LC/Q-TOF Author David L. Wong Agilent Technologies, Inc. Introduction Monoclonal antibodies (mAbs) and their derivatives represent a very complex but important class of biopharmaceutical…

Agilent BioHPLC Columns Characterization of NIST Monoclonal Antibody Critical Quality Attributes Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…