Automated method optimization for drug-to-antibody ratio determination using hydrophobic interaction chromatography

Analysis of Cysteine-Linked Antibody Drug Conjugates Using Hydrophobic Interaction Chromatography on the Agilent 1260 Infinity II Bio-inert LC Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Hydrophobic interaction chromatography (HIC) is frequently used for the Agilent Technologies, Inc. determination…

Biopharma High-sensitive multi-attribute analysis of ADCs under native conditions by using an online multiple heart-cut 2D-LC-HRAM mass spectrometry system Frank Steiner1, Maria Gruebner1, Xuepu Li2, Xiaoxi Zhang2, Min Du3 1Thermo Fisher Scientific, Germering, Germany, 2Thermo Fisher Scientific, Shanghai, China, 3Thermo…

Consumables Workflow Ordering Guide Improving the Quality of Antibody Drug Conjugates by Orthogonal Analytical Methods Antibody Drug Conjugates Antibody-drug conjugates (ADCs) represent a new generation of targeted biotherapeutics that make up a rapidly growing segment of the drug discovery pipeline.…

Application Note Biopharma High Salt—High Reproducibility Analysis of antibody drug conjugates using hydrophobic interaction chromatography with the Agilent 1290 Infinity II Bio LC System Response (mAU) ×101 D2 3.4 3.2 3.0 2.8 2.6 2.4 2.2 2.0 1.8 1.6 1.4 1.2…