Cursory Analysis of Nucleic Acid Components in Adeno-Associated Virus (AAV) Samples Using Anion-Exchange Chromatography (AEX)

[ APPLICATION NOTE ] Anion-Exchange Chromatography for Determining Empty and Full Capsid Contents in Adeno-Associated Virus Hua Yang, Stephan Koza, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ Empty and full AAV capsid separation Recombinant adeno-associated…

CONSUMABLES GUIDE BOOK Characterizing AAVs Chromatographic methods to measure heterogeneity and critical quality attributes CONSUMABLES GUIDE BOOK A Changing Industry Attribute Testing The advent of FDA approved gene therapies brings An AAV vectored therapy contains multiple types of biomolecules compromised…

Salt gradient separation of nucleic acid components in Adeno-Associated Virus using anion exchange chromatography Sissi White1, Estee Toole2, Kenneth Thompson3, Ken Cook4, Min Du1. 1Thermo Fisher Scientific, Lexington, MA, US; 2Thermo Fisher Scientific, West Palm Beach, US, 3Thermo Fisher Scientific,…

Comprehensive AAV Characterizations Using HRMS: Peptide Mapping, Capsid Proteins, Intact ssDNA, and Intact Virus T1430-09-53 Hua Yang, Ying Qing Yu, Stephan Koza, Suraj Dhungana, Ann Gray Waters Corporation, 34 Maple Street, Milford, Massachusetts, USA CONTACT INFORMATION: [email protected] PURPOSE RESULT(S) CONCLUSION(S)…