HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual eluent generation cartridges

HPAE-PAD Analysis of N-linked Oligosaccharides from Glycoproteins Using Dual Eluent Generation Cartridge Mode Beibei Huang, Lillian Chen, Joachim Weiss, and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA , USA, 94085 ABSTRACT Data Analysis Purpose: To demonstrate Dual Eluent Generation Cartridge…

Deanna Hurum, Lipika Basumallick, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Prostate-Specific Antigen (PSA), Transferrin, N-Glycans, O-Glycans, Dionex CarboPac Column Introduction Changes in protein glycosylation are frequently studied in cancer research to identify potential biomarkers. Factors…

TECHNICAL NOTE 72264 HPAE-PAD N-linked oligosaccharide profiling of IgG Authors Sachin Patil, Deanna Hurum, Lipika Basumallick, and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Goal To develop an HPAE-PAD method to profile the major neutral and charged asparagine-linked (N-linked) oligosaccharides…

Application Note 215 Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column Introduction High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a widely used technique for determining an extensive set of carbohydrates including, but…