Intact mass analysis of monoclonal antibody charge variants by multi heart- cut 2D-LC/MS coupling ion-exchange and reversed-phase chromatography

Application note | 004167 Biopharma Charge variant analysis of cysteine-linked antibody-drug conjugates using an online multiple heart-cut 2DSCX-SEC-LC HRAM MS approach Authors Application benefits Xuepu Li¹, Xiaoxi Zhang¹, Maria Grübner², • The online multiple heart-cut two-dimensional liquid chromatography (2D-LC) system…

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…

Biopharma High-sensitive multi-attribute analysis of ADCs under native conditions by using an online multiple heart-cut 2D-LC-HRAM mass spectrometry system Frank Steiner1, Maria Gruebner1, Xuepu Li2, Xiaoxi Zhang2, Min Du3 1Thermo Fisher Scientific, Germering, Germany, 2Thermo Fisher Scientific, Shanghai, China, 3Thermo…

Application note | 004276 Biopharma Monitoring monoclonal antibody bioproduction processes with 2D-LC-UV Author Application benefits Maria Grübner, Thermo Fisher Scientific, • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of cell culture samples enables titer determination and purification for subsequent…