From HPLC to UHPLC: What are the Instrumental Requirements and Pitfalls?

WHITE PAPER 72711 An instrument parameter guide for successful (U)HPLC method transfer Authors Carsten Paul, Maria Grübner, Michael Heidorn, Matthias Krajewski, Sabrina Patzelt, Thomas Piecha, and Frank Steiner Thermo Fisher Scientific, Germering, Germany Keywords UHPLC, HPLC, USP <621>, gradient delay…

TECHNICAL NOTE 70828 A universal tool for method transfer from HPLC to UHPLC Authors Introduction Holger Franz and Susanne Fabel; Thermo Fisher Scientific, Germering, Germany With the commercialization of ultra high performance liquid chromatography (UHPLC), there has been a continuing…

From HPLC to UHPLC: Method Transfer, What Should I Pay Attention to? Dr. Frank Steiner Thermo Fisher Scientific, Germering/Germany The world leader in serving science A Well-Known Situation in the Lab • Why does a chromatogram recorded on system X……

Holger Franz and Alexandra Manka Thermo Fisher Scientific, Germering, Germany Key Words HPLC Method Transfer, Agilent 1100 System, UltiMate 3000 System, Gradient Application, Gradient Delay Volume Adjustment, Peak Dispersion, Peak Resolution Goal The goal of this Technical Note is to…