HPAE-FLD Method for Separation of 2-Aminobenzamide (2-AB)-Labeled Oligosaccharides from Human α1 Acid-Glycoprotein and Bovine Fetuin

APPLICATION UPDATE Sachin Patil and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA Goal To demonstrate improved chromatographic separation achieved using a Thermo Scientific™ Dionex™ CarboPac™ PA200 column for the HPAE-FLD method described in proposed United States Pharmacopeia (USP) General Chapter…

TECHNICAL NOTE 70671 Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Author: Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Technical Note 20, monosaccharide, alditol, glycan, oligosaccharide, sialic acid, sugar Goal Update Dionex Technical Note 20 to…

TECHNICAL NOTE 70671 Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Author: Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Technical Note 20, monosaccharide, alditol, glycan, oligosaccharide, sialic acid, sugar Goal Update Dionex Technical Note 20 to…

Technical Note 36 Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD INTRODUCTION Analysis of protein glycosylation is an important part of glycoprotein characterization. Oligosaccharides can be linked to a protein through a serine or a threonine as O-linked glycans,…