Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System

Mauro De Pra and Carsten Paul Thermo Fisher Scientific, Germering, Germany Ap plica t ion Note 1 1 3 1 Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System Key Words Biotherapeutics, Biosimilars, Intact Proteins, Stability, mAbs…

Michael Menz, Mauro De Pra, Evert-Jan Sneekes Thermo Fisher Scientific, Germering, Germany Te ch ni cal N ote 1 7 3 Influence of Column Temperature on Reversed-Phase Chromatography of an Intact Antibody Key Words Monoclonal Antibodies, MabPac RP Column, Biocompatible…

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…

APPLICATION NOTE 72599 Simultaneous analysis of monoclonal antibodies using a novel dual-channel UHPLC instrument and orthogonal chromatography Authors Nicola McGillicuddy,1 Amy Farrell,1 Sara Carillo,1 Martin Samonig,2 Jonathan Bones1 1 National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland 2…