Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography

Application Update 141 Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform N-acetylneuraminic acid (Neu5Ac) and Nglycolylneuraminic acid (Neu5Gc) are sialic acids that occupy terminal positions on many mammalian glycoprotein…

Application Update 180 Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD INTRODUCTION Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and metabolism.1 Although over 50 natural sialic acids have been identified,2 two forms are commonly determined…

Application Update: 181 Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD Deanna Hurum and Jeff Rohrer Introduction • Neuraminic Acids • Anion Exchange • Pulsed Amperometric Detection • CarboPac PA20 Fast Sialic Acid Column • High-Throughput Analysis Glycoprotein sialylation…

APPLICATION NOTE 74121 Determination of glycoprotein sialic acid composition using HPAE-PAD in dual eluent generation cartridge mode Authors: Jingli Hu and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Dionex CarboPac PA20-1 mm column, Dionex ICS-6000 HPIC system, electrochemical detection,…