Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct

Technical Note 50 Determination of the Amino Acid Content of Peptides by AAA-Direct INTRODUCTION EQUIPMENT ™ The AAA-Direct system separates amino acids on a high performance anion-exchange column and directly detects them by integrated amperometry.1, 2 AAA-Direct has advantages over…

Application Note 150 Determination of Amino Acids in Cell Cultures and Fermentation Broths INTRODUCTION Cell cultures and fermentation broths are used in the manufacture of biotherapeutics and many other biological materials produced using recombinant genetic technology. Broths are also used…

Application Note 163 Determination of Protein Concentrations Using AAA-Direct™ INTRODUCTION Dye-binding assays such as bicinchoninic acid (BCA), Lowry, and Bradford (Coomassie) are the most common methods to determine total protein. The BCA assay depends on Cu (II) reduction to Cu…

Technical Note 55 Screening of Sample Matrices and Individual Matrix Ingredients for Suitability in AAA-Direct™ INTRODUCTION All chromatographic techniques are susceptible to chemical interferences. Problems associated with chemical interferences include coelution with analytes, column contamination, shifts in retention times, and…