Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Agilent Cary Eclipse

Minimizing photobleaching of Blue Fluorescent Protein (BFP) using the Agilent Cary Eclipse fluorescence spectrophotometer Application Note Author Introduction Paul Gavin*, Mark Prescott*, Ph.D, Daren J. Fyfe, Ph.D and Jeffrey J. Comerford**, Ph.D The term photobleaching refers to loss of fluorescence…

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Agilent Cary Eclipse Application Note Author Paul Gavin# and Mark Prescott#, Ph.D Daren J. Fyfe, Ph.D* #Department of Biochemistry and…

Sensitive. Accurate. Flexible. Agilent Cary Eclipse fluorescence spectrophotometer Agilent Cary Eclipse Fluorescence Spectrophotometer Agilent is your premier resource and partner for molecular spectroscopy. The world-renowned Cary product line, encompasses FTIR, UV-Vis-NIR, and fluorescence instruments, offering you a comprehensive range of…

Fluorescence measurement of hybridization between quencher (DABCYL) labelled PNA probes and a fluoresceine labelled DNA using the Fluorescence BioMelt Package Application Note Author Katherine Lighton, Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia. Mark J. Fiandaca, Boston Probes, Bedford, Massachusetts 01730,…