Automated High-Throughput N-Glycan Labelling and LC-MS Analysis for Protein A Purified Monoclonal Antibodies

[APPLICATION NOTEBOOK] At-line Solutions for Upstream Bioprocessing Application Notes [APPLICATION NOTEBOOK] Introduction Bioprocessing harnesses the power of living cells to manufacture valuable products such as biological drugs for clinical use. The introduction and use of biologics has revolutionized biotherapeutics to…

Application Note A Modified GlycoWorks Procedure With Enhanced Detection Sensitivity Caitlin M. Hanna, Stephan M. Koza, Steve Shiner Waters Corporation Abstract Here, we present a modified GlycoWorks™ RapiFluor-MS™ procedure that enhances FLR and MS sensitivity for the detection of low-abundance…

Application Note Automated High-Throughput AnalyticalScale Monoclonal Antibody Purification Using Production-Scale Protein A Affinity Chromatography Resin Stephan M. Koza, Caitlin M. Hanna, Albert H. W. Jiang, Ying Qing Yu Waters Corporation Abstract An automated analytical-scale Protein A affinity based purification of…

Application Note Automated RapiFluor-MS Labeled Glycan Sample Preparation for Disulfide-Rich Glycoprotein Yamin Htet, Stephan M. Koza Waters Corporation Abstract N-glycans are routinely monitored for the quality of biotherapeutics because they affect the safety and efficacy of many therapeutic proteins. Current…