Oligonucleotide characterization using μPAC columns in ion-pairing reversed-phase high performance liquid chromatography mode

[ TECHNOLOGY BRIEF ] Factors Influencing Diastereomer Separations in Oligonucleotide Analysis Robert E. Birdsall, Martin Gilar, and Ying Qing Yu Waters Corporation, Milford, MA, USA Separation of diastereomers can be influenced through the type (“weak” vs. “strong”) and concentration of…

Product spotlight | 000474 Peptide separation Peptide separation on μPAC HPLC columns Part 1: Cytochrome c tryptic digest Goal Keywords Demonstrate the gain in chromatographic performance that can be achieved for protein Proteomics, tryptic digest, nano liquid tryptic digest samples…

Separation of Impurities from a Fully Phosphothioated and 2’-O-methylated RNA Using Ion-Pair ReversedPhase Chromatography Julia Baek, Jim Thayer, Shanhua Lin, Xiaodong Liu, Robert Van Ling, Thermo Fisher Scientific, 1228 Titan Way, Sunnyvale, CA, USA, 94085 Effect of Ion-Pair Reagents Methods:…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…