Interlaboratory study of an optimised peptide mapping workflow using automated trypsin digestion for monitoring product quality attributes

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

CUSTOMER APPLICATION NOTE 73826 Peptide mapping of challenging monoclonal antibodies Authors: Dan Bach Kristensen1, Trine Meiborg Sloth1, Martin Ørgaard1, Krisztina Radi2 Symphogen, Ballerup, Denmark Thermo Fisher Scientific, Hemel Hempstead, UK 1 2 Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modifications,…

APPLICATION NOTE 72916 A high-resolution accurate mass multi-attribute method for critical quality attribute monitoring and new peak detection Authors Haichuan Liu1, Royston Quintyn2, John Rontree3 Thermo Fisher Scientific 1 San Jose, CA 2 West Palm Beach, FL 3 Hemel Hempstead,…

Application Note Biotherapeutics Quantitation of Chemical-Induced Deamidation and Oxidation on Monoclonal Antibodies Using Agilent 6545XT AdvanceBio LC/Q-TOF and Agilent MassHunter BioConfirm Software Author Linfeng Wu Agilent Technologies, Inc. Santa Clara, CA, USA Introduction Modifications such as asparagine (Asn) deamidation, aspartate…