Determination of Proteins and Carbohydrates by 2D HPLC (RPLC and HILIC) with Charged Aerosol and Ultraviolet Detection

directly by UV absorbance detectors. HPLC with various detection techniques has been used for carbohydrate analysis, such as IR, mass spectrometry, UV or fluorescence following derivatization. Although methods utilize derivatization improve the chromatographic resolution and detector sensitivity, they can lead…

APPLICATION NOTE 122 The determination of carbohydrates, alcohols, and glycols in fermentation broths Authors Valoran Hanko and Jeffrey S. Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords Dionex CarboPac MA1 column, Dionex CarboPac PA1 column, pulsed amperometric detection, PAD, high-performance…

Table of Contents Introduction Analytical Technologies Chromatography Analysis of Carbohydrates Corn Syrup Dietary Fiber Lactose and Lactulose Maltodextrins Sialic Acid Beverages Honey Chromatography for Foods and Beverages References Carbohydrates Analysis Applications Notebook Novel, Selective, Sensitive Analytical Methods Table of Contents…

Table of contents Summary Overview Excipient function Measurement Charged Aerosol Detector Universal detection Uniform response Working principles (U)HPLC Systems with CAD Columns choice Excipients overview Adjuvants Amino acids Carbohydrates Counterions Nonionic surfactants Polyethylene glycol Proteins and excipients HPLC-Charged Aerosol Detection…