The importance of correct UHPLC instrument setup for protein aggregate analysis by size-exclusion chromatography

APPLICATION NOTE 72362 Lifetime stability of size exclusion chromatography columns for protein aggregate analysis Authors Amy Farrell1, Craig Jakes1, Alexander Ley2, Mauro De Pra2, Frank Steiner2, and Jonathan Bones1 The National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland…

Chromatographic Workflows for Biopharmaceutical Characterization Dr. Ken Cook European Bioseparation Sales Support Expert Thermo Fisher Scientific, Hemel Hempstead/Germany The world leader in serving science Fulfilling the Needs of Biotherapeutic Characterization Charge variant screening Peptide mapping Released glycan analysis Aggregate screening…

APPLICATION NOTE Amy Farrell1, Jonathan Bones1, and Ken Cook2 1 NIBRT, Dublin, Ireland; 2 Thermo Fisher Scientific, Hemel Hempstead, UK Key words MAbPac SEC-1, monoclonal antibody, mAb, aggregation analysis, biotherapeutics, size-exclusion chromatography Goal Analysis of protein aggregation of five important…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…