Separation of Native Monoclonal Antibodies and Identification of Charge Variants: Teamwork of the Agilent 3100 OFFGEL Fractionator, Agilent 2100 Bioanalyzer and Agilent LC/MS Systems

BioPharma Applications Compendium AGILENT APPLICATIONS FOR BIOPHARMACEUTICAL DISCOVERY, DEVELOPMENT AND QA/QC s s s s P L A Y R P L A Y R Q S M A Y R Q S M N N F S M N…

Monoclonal Antibody Charge Heterogeneity Analysis by CZE and CZE/MS Comparison of charge variant profiles of Rituximab Innovator and Biosimilar mAbs Application Note Biopharmaceutical Author Introduction Suresh Babu C.V. During biosysnthesis, various post-translational modifications (PTMs) will lead to charge heterogeneity in…

Charge Heterogeneity Analysis of Intact Infliximab Using CESI-MS and Neutral OptiMS Cartridge Fang Wang1, Tingting Li1, Esme Candish2 1 SCIEX, Brea, CA; 2SCIEX, Framingham, MA; Key Features • Capillary zone electrophoresis (CZE) separation and Mass spectrometric (MS) identification of charge…

Native ion exchange chromatography directly coupled to Orbitrap mass spectrometry allows surface charge discrimination and online detection of intact proteins Aaron O. Bailey1, Guanghui Han2, Wilson Phung2, Paul Gazis1, Jennifer Sutton1, Wendy Sandoval2, Jonathan L. Josephs1 1Thermo Fisher Scientific, 355…