Improving UPLC-FLR-MS N-glycan Analysis of Disulfide-rich Fusion Proteins Through Optimization of Sample Preparation

Application Note Automated RapiFluor-MS Labeled Glycan Sample Preparation for Disulfide-Rich Glycoprotein Yamin Htet, Stephan M. Koza Waters Corporation Abstract N-glycans are routinely monitored for the quality of biotherapeutics because they affect the safety and efficacy of many therapeutic proteins. Current…

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent Matthew A. Lauber,1 Darryl W. Brousmiche,1 Zhengmao Hua,1 Stephan M. Koza,1 Ellen Guthrie,2 Paula Magnelli,2 Christopher H. Taron,2 Kenneth J. Fountain1 1 Waters Corporation,…

The Road to Glycan Analysis Without Compromise WCBP 2015 Waters Technical Seminar Jan 27, 2015 ©2015 Waters Corporation Washington, DC 1 Today’s Agenda  Overview and Introduction  RapiFluor-MS N-Glycan Labeling: A breakthrough technology for released glycan LC and MS…