Performance Comparison of Binary Ultra High Performance Liquid Chromatography Systems from Multiple Vendors for Methods Which Utilize Long, Shallow Gradients

[ APPLICATION NOTE ] Performance of the ACQUITY UPLC I-Class PLUS System for Methods which Employ Long, Shallow Gradients Jennifer Simeone, Paula Hong, and Patricia R. McConville Waters Corporation, Milford, MA, USA APPLICATION BENEFITS INTRODUCTION Repeatable retention time delivery One…

PEPTIDE MAPPING: BEST PRACTICES FOR GENERATING RELIABLE AND ROBUST LIQUID CHROMATOGRAPHY METHODS Jennifer Simeone, Paula Hong Waters Corporation, Milford, MA, USA One of the goals of liquid chromatography method development is to develop a method which provides reliable and reproducible…

[ APPLICATION NOTE ] Enhancing Robustness for Biopharmaceutical Separations Using the Waters ACQUITY UPLC PLUS Series Ximo Zhang, Robert Birdsall, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ INTRODUCTION Enhanced reproducibility for both With effective…

Evaluation of Pump Performance for Long Shallow Gradient Peptide Mapping Analysis Andrew Steere, Norris Wong, Paula Hong Waters Corporation, Milford, MA, 01757 RESULTS AND DISCUSSION INTRODUCTION Peptide mapping is a critical tool in biopharmaceutical analysis for characterization and impurity testing.…