SEC Coupled to High-Resolution Mass Spectrometry for Detailed Characterization of mAbs and ADCs

Application Note Biologics Efficient Monoclonal Antibody and Antibody Drug Conjugate Desalting Prior to Mass Spectrometry Using AdvanceBio Desalting-RP Cartridges Authors Isabel Vandenheede, Pat Sandra, and Koen Sandra Research Institute for Chromatography (RIC) President Kennedypark 26 B-8500 Kortrijk Anne Blackwell and…

Agilent-NISTmAb Intact & Subunit Analysis Using Reversed Phase Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…

Characterize Fab and Fc Fragments by Cation-Exchange Chromatography Application Note Biologics and Biosimilars Authors Abstract Isabel Vandenheede, Emmie Dumont, This application note describes the use of the Agilent Bio MAb weak Pat Sandra, and Koen Sandra cation-exchange column in measuring…

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…